WebApr 13, 2024 · To characterize host components involved in immunity against fungi, we performed a protein pull-down with the biotinylated β-glucan laminarin. Thereby, we identified a glycoside hydrolase family 81-type glucan-binding protein (GBP) as the major β-glucan interactor. Mutation of GBP1 and its only paralogue GBP2 in barley led to … WebFor biotin-based in vitro pull-down assay, GFP, PYL5 and eight polyketide cyclase-like proteins fused at the C-terminus to the HPB tag were purified from N. benthamiana as described above and stringently washed to remove bound endogenous metabolites [four washes with extraction buffer (150 m m NaCl, 50 m m TRIS-HCl pH 7.5, 10% glycerol, …
Optimized small‐molecule pull‐downs define MLBP1 as an …
WebRNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with RNase-free DNase I (Promega) and purified with RNeasy Mini Kit (QIAGEN). 2. Biotin-HRP Northern blot was followed by manufacture’s manual (NorthernMax kit, WebFeb 10, 2024 · Here, we demonstrate the step-by step optimization of pull-down for IRP (iron-responsive-element-binding protein) with a 5'-biotinylated stem-loop IRE (iron-responsive element) RNA, HuR, and AUF1 with an AU-rich RNA element and Nrf2 binding to an antioxidant-responsive element (ARE) enhancer in the human ferritin H gene. simulated jewelry meaning
Aureusidin derivative CNQX inhibits chronic colitis inflammation …
WebFeatures of the Biotinylated Protein Interaction Pull-Down Kit: • Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions. • Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein … Features of the Biotinylated Protein Interaction Pull-Down Kit: • Provides a … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebMany methods exist for investigating protein-protein interactions. The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins … WebIdentifying unknown protein binding partners using mass spectrometry (MS)-based proteomics has evolved into an indispensable strategy in drug discovery. ... The biotin … simulated instrument flight