WebNorthernMax™ High Stringency Wash Buffer Catalog number: AM8674 Related applications: Nucleic Acid Gel Electrophoresis & Blotting Technical Support Customer Service Save to list Add to cart Request bulk or custom quote Product Overview … The equivalent of 0.1X SSC or SSPE, this Ambion® wash buffer is functionally … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebFormamide is a potential carcinogen Stringent wash solution: typically, use a final concentration of 20% formamide and 0.1x SSC for high stringency (40 ml 100% formamide and 1 ml 20x SSC made up with water to 200 ml) or 2x SSC (40 ml 100% formamide and 20 ml 20x SSC made up to 200 ml) for low stringency (Note 2) METHODS

NorthernMax™ High Stringency Wash Buffer - Thermo …

WebWash the membrane in low-stringency washes to remove hybridization solution and unhybridized probe, and high-stringency washes to remove partially hybridized molecules. Follow manufacturer directions for chemiluminescent detection. Western Blot WebIt is illegal to put a structure in a buffer or do any type of construction. Buffer restrictions even apply to septic systems, swimming pools, hard-surface pathways, and grading the … エトポシド 尿

Frontiers Double In situ Hybridization for MicroRNAs and mRNAs …

WebComposition: Water 68.93%, Ammonium Acetate 23.28%, Hydrochloric Acid 7.79% Boiling Point: 100 to 118°C Density: 1.07 Melting Point: 0°C Color: Colorless liquid Physical State: … WebDec 15, 1999 · The low stringency immunoprecipitations were washed three times with low stringency buffer and co-immunoprecipitated proteins eluted by incubation in high stringency buffer. The eluted proteins were … WebThe Invitrogen ™ NorthernMax High Stringency Wash Buffer (Cat. No. AM8674) is a proprietary formulation equivalent to 2X SSC or 2X SSPE wash buffers. Contents and … エトフ バッグ公式