WebDec 17, 2016 · Drug-induced phospholipidosis caused by combinations of common drugs in vitro.药物引起phospholipidosis引起的常见药物在体外 ... combination limitmea- surement mistakes. cellswere further24 concomitantviability detection, 11 PrestoBlueCellViability Reagent (PrestoBlue) (Invitrogen, Carlsbad, US) were added eachwellafter 22 hto ... WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform.

HCS LipidTOX™ Red Phospholipidosis Detection Reagent …

WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of … WebJul 16, 2015 · For phospholipid staining, the cells were treated with 400 μM palmitate containing 1X LipidTOX Red phospholipidosis detection reagent. For Immunostaining with an anti-calnexin antibody, the cells were fixed in 4 % paraformaldehyde in PBS. The fixed cells were rinsed with PBS and incubated in blocking solution (5 % goat serum, and 0.001 … sid meier’s civilization v

Identification of Drugs Inducing Phospholipidosis by Novel in vitro ...

Webreagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials. Dispose of the reagents in compliance with all pertaining local … WebHCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is … sid meier railroad tycoon