Thy1 cre

Author: lcbo

August undefined, 2024

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Tamoxifen-induced knockdown of the mitochondrial calcium ... - PubMed

Webb20 nov. 2012 · The lines highlighted here, Thy1-cre, Grik4-cre, GFAP-cre and Cyp39a1-cre, were created using transgenic approaches, and as such the observed unexpected activity could result from integration site ... Webb品系名： C57BL/6JSmoc- Them1 (2A-tdTomato)Smoc. 品系状态：胚胎冻存目录号：NM-KI-210124. 应用领域：该小鼠在Th基因座表达报告基因 ( tdTomato)。. 该报告基因在儿茶酚胺能细胞中表达，可用于研究多巴胺能细胞功能、帕金森病和其他神经退行性疾病。. … fee2main

Optimizing Nervous System-Specific Gene Targeting with Cre …

WebbThy1是锥体神经元的特异性启动子，在前脑、海马、杏仁核、丘脑及视网膜等区域均有丰 … Webb8 apr. 2024 · The Dlx5/6-Cre line (Tg(dlx5a-cre)1Mekk) (Zerucha et al., 2000) has been used in over 70 papers to specifically target forebrain interneurons (Mouse Genome Informatics [MGI] database; Bult et al., 2024).We combined this transgene with the Clstn3 f/f (B6-Clstn3 tm1Amcr /J) allele (Pettem et al., 2013) and then crossed Clstn3 f/f; Dlx5/6 … Webb012708 STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ These SLICK-H transgenic mice … defaultservicemanager addservice

Thy1 cre

Thy1 marks a distinct population of slow-cycling stem cells in the

WebbA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields WebbIn Thy1-Cre mice, Cre activity is, amongst others, present in hippocampal neurons (Figure 2B), which is important since the hippocampus is highly affected by AD pathology. Thy1-Cre mice...

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WebbPCR步骤. 5,12000rpm离心10min，取上清400μl到另一1.5mlEP管内。. b取“另一1.5mlEP管”时，选择盖子内部正常无多余部分，以免第6步的絮状沉淀挂在多余部分。. 6,向每只EP管内加入2倍体积的无水乙醇800μl，轻轻翻转几次混匀，可见絮状DNA沉淀（若看不见沉淀可在 … WebbThe Thy1 Cre ERT2-eYFP and MEC17 fl/fl mice were mated to produce Thy1 Cre ERT2-eYFP::MEC17 fl/fl mice. Tamoxifen (75 mg/kg body weight; 10,540–29-1, Sigma) was dissolved in corn oil and intragastrically administered once every 24 h for five consecutive days to induce gene recombination in 4-week-old Thy1 Cre ERT2-eYFP mice.

Webb7 feb. 2024 · Tg (Thy1-cre)703Vaw. Mutation details : A 1.1kb Cre-containing cDNA … Webb22 maj 2024 · Relative to TMX/Thy1-cre/ERT2-eYFP controls, HI-induced sensorimotor deficits, forebrain neuron loss and mitochondrial damage were decreased for conditional MCU knockout mice. MCU knockdown by siRNA-induced silencing in cortical neuron cultures also reduced cell death and mitochondrial respiratory deficits following oxygen …

Webb22 maj 2024 · To examine if the MCU is also involved in hypoxic/ischemic (HI) brain … Webb11 feb. 2024 · utilized as an alternative to these pre-existing Thy1-Cre Tg mice. Certain sets of cells in the intestine, liver , and kidney did not fluoresce in Dre reporter mice despite the use of the ...

Webb10 apr. 2024 · Tigit-Cre-ERT2 mice were established using 129 ES cells in which the IRES-EGFP-Cre-ERT2 cassette was inserted into the 3′ untranslated region of Tigit using CRISPR/Cas9 in our facility. Tigit-Cre-ERT2 mice were backcrossed with C57BL/6 mice seven times and with Rosa26-tdTomato mice (#:007914; Jackson mouse, strain) for the …

Webb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. defaultservlethandlerconfigurer springbootWebbTg (Thy1-cre/ERT2,-EYFP)HGfng. Mutation details : The transgene was designed with a … default session timeout in angular10WebbSurprisingly, both Thy1-Cre/HDAC4(-/-) mice, in which HDAC4 is ablated in neurons of the cortex and hippocampus, as well as Nes-Cre/HDAC4(-/-) mice, in which HDAC4 is ablated in neural progenitor cells of the CNS, appear normal at birth, have normal body weight, are fertile, and perform normally in locomotor activity assays. default services windows 10